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EIF4A1 (Succinyl-K309) Antibody#SAB610

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Product Detail

Product NameEIF4A1 (Succinyl-K309) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was purified from rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

ApplicationsWB

Species ReactivityHuman

SpecificityEIF4A1(Succinyl-K309) Antibody detects endogenous levels of eIF4A1 only when succinylated at K309.

Immunogen DescA synthesized peptide derived from human eIF4A1 around the succinylated site of K309.

ConjugateUnconjugated

Other NameseIF-4A-I,eIF4A-I,DDX2A, EIF4A

Uniprot P60842

Gene ID 1973

Sdspage MW46kDa

Concentration1 mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.

StorageStore at --20˚C/1 year

Application Details
Western Blot: 1/500 - 1/2000
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

If you have published an article using product SAB610, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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