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GPX4 (Phospho-Ser104) Antibody#SAB712P

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Product Detail

Product NameGPX4 (Phospho-Ser104) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsCustom antibody

Species ReactivityHu

Immunogen DescImmunogen Type:Peptide-KLHImmunogen Description:Peptide sequence around phosphorylation site of serine104 ( C-AGYNVKFDMF-S(p)-KI) derived from Human GPX4.

Target NameGPX4

Other NamesPhospholipid hydroperoxide glutathione peroxidase GPX4,PHGPx,Glutathione peroxidase 4 ,GPX4

Uniprot P36969

Gene ID 2879

Calculated MW20/22kDa

Sdspage MW33 kDa

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
All lanes: GPX4 (Phospho-Ser104) Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 10% serum for 30min whole cell lysates
Lane 3 : C6 whole cell lysates
Lane 4 : C6 treated with 10% serum for 30min whole cell lysates
Lane 5 : MCF7 whole cell lysates
Lane 6 : MCF7 treated with 10% serum for 30min whole cell lysates
Lane 7 : MCF7 treated with Lambda Protein Phosphatase for 30min whole cell
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 20/22 kDa
Observed band size: 33 kDa
Exposure time: 10 seconds

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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