Product Detail
Product NameHA-Tag Rabbit Polyclonal Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
SpecificityThe antibody detects HA-tagged proteins overexpressed in cells. The antibody recognizes the HA-tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected cells.
Immunogen TypePeptide
Immunogen DescPeptide sequence (Y-P-Y-D-V-P-D-Y-A).
Target NameHA-Tag
ConjugateUnconjugated
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: kd
Western blotting: 1:500~1:1000
Epitope tags are artificial epitopes useful for the labeling and detection of proteins. Epitope tags - short amino acid sequences are 'fused' to the N- or C-terminus of the protein.
Field, J. et al. (1988) Mol. Cell. Biol. 8, 2159
If you have published an article using product T501, please notify us so that we can cite your literature.
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