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Avi Tag Mouse Monoclonal Antibody(6E3)#T517

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Product Detail

Product NameAvi Tag Mouse Monoclonal Antibody(6E3)

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsWB

SpecificityThe Avi-Tag Mouse Monoclonal Antibody detects Avi-Tag recombinant protein.

Target NameAvi Tag

ConjugateUnconjugated

Other NamesAvi Tag antibody

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 51% glycerol.

StorageStore at -20˚C

Application Details
Western blotting: 1:5000~1:10000
Western blot analysis of Avi-Recombinant protein, using #T517 diluted at 1) 1:5,000 2) 1:10,000
The Avi tag is a biotin-acceptor peptide1, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain.

If you have published an article using product T517, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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