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mCherry -Tag Rabbit Polyclonal Antibody#T521

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Product Detail

Product NamemCherry -Tag Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAffinity purification using immunogen.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe mCherry tag antibody can recognize mCherry tag fusion proteins.

Target NamemCherry-Tag

ConjugateUnconjugated

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details

Western blotting: 1:5000~1:10000

1ug mCherry fusion protein+ Primary antibody #T521 dilution at 1:5,000 (lane 1) and 1:10,000 (lane 2).

mCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587nm and 610nm, respectively. It is resistant to photobleaching and is stable. mCherry is sometimes preferred to other fluorophores due to its colour, as well as its photostability compared to other monomeric fluorophores.

If you have published an article using product T521, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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