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S-Tag Mouse Monoclonal Antibody#T611

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Product Detail

Product NameS-Tag Mouse Monoclonal Antibody

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG1

PurificationAffinity-chromatography

ApplicationsWB

SpecificityS- Tag antibody can recognize C-terminal, internal, and N-terminal S-tagged proteins.

Immunogen TypePeptide-KLH

Immunogen DescA synthetic peptide KETAAAKFERQHMDS coupled to KLH.

Target NameS-Tag

ConjugateUnconjugated

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20 °C.Stable for 12 months from date of receipt

Application Details

 WB: 1:3000~1:10000

2ug S-Tag fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000

 S-tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A). If RNase A is digested with subtilisin, a single peptide bond is cleaved, but the resulting two products remain weakly bound to each other and the protein, called ribonuclease S, remains active although each of the two products alone shows no enzymatic activity. The N-terminus of the original RNase A, also called S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag.The amino acid sequence of the S-tag is: KETAAAKFERQHMDS conjugated to KLH. S- Tag antibody can recognize C-terminal, internal, and N-terminal S-tagged proteins.

If you have published an article using product T611, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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