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Mouse Anti-Human CD14,PE Conjugated mAb

28288
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  Catalog No. Package Size Price
28288-1 25 Tests $119.00
28288-2 50 Tests $288.00
28288-3 100 Tests $368.00

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Description
  • Product Name Mouse Anti-Human CD14,PE Conjugated mAb
  • Host Species Mouse
  • Clonality Monoclonal
  • Clone No. 3A8
  • Isotype Mouse IgG2a, κ
  • Applications FC
  • Species Reactivity Hu
  • Specificity This antibody recognizes human CD14 in FACS.
  • Immunogen Description Human peripheral blood mononuclear cells
  • Formulation Lyophilized from a 0.2μm filtered solution in phosphate buffered saline (PBS) and reconstitute with sterile PBS .
  • Storage Store protected from light at 2-8˚C. Do not freeze. The expiration date is indicated on the vial label.
Application Details

Format:Antibodies are supplied in buffer containing stabilizer and 0.05% sodium azide
Preparation:This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with human CD14 Recombinant Protein). The monoclonal antibody was purified from tissue culture supernatant or ascites by protein G affinity chromatography.
Product Notices:This reagent has been pre-diluted for use at the recommended volume per test. We typically use 1 106 cells in a 100-?l experimental sample (per test). An isotype control should be used at the same concentration as the antibody of interest.

Images
  • Mouse Anti-Human CD14,PE Conjugated mAb - SAB | Signalway Antibody

    Flow cytometric analysis of CD14 expression on human peripheral blood mononuclear cells (PBMCs). PBMCs were stained with either mouse IgG2a, κ Isotype control or mouse anti-human CD14 antibodies conjugated to PE. Fluorescence histograms showing the expression of CD14 (or Ig Isotype control staining) were derived from events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a Beckman FC 500 Flow Cytometer System.

Product Description
Cluster of differentiation 14 (CD14) is a member of the CD system. It takes its name from its inclusion in the CD molecule surface marker proteins.CD14 exists in two forms: a form anchored into the membrane or a soluble form. CD14 was found expressed in macrophages, neutrophil granulocyte and dendritic cells. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS).CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns such as lipoteichoic acid.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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