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DNA Polymerase gamma antibody

22870
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  Catalog No. Package Size Price
22870 100ul $390.00

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Description
  • Product Name DNA Polymerase gamma antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Purified by antigen-affinity chromatography.
  • Applications WB IHC IF
  • Species Reactivity Hu
  • Immunogen Type Recombinant Protein
  • Immunogen Description Recombinant protein fragment contain a sequence corresponding to a region within amino acids 779 and 1191 (P54098) of DNA polymerase gamma
  • Target Name DNA Polymerase gamma
  • Accession No. NCBI Gene ID: 5428
    NCBI mRNA#: NM_002693
    NCBI Protein#: NP_002684
  • Concentration 0.67mg/ml
  • Formulation Supplied in 1XPBS, 1%BSA, 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
  • Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details

Predicted MW: 140kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200
 

Images
  • DNA Polymerase gamma antibody - SAB | Signalway Antibody

    Sample (30 ug of whole cell lysate)
    A: MCF-7
    5% SDS PAGE
    Primary antibody diluted at 1: 1000

  • DNA Polymerase gamma antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded Skeletal Muscle , using DNA polymerase gamma antibody(10 ug/ml).

  • DNA Polymerase gamma antibody - SAB | Signalway Antibody

    Immunofluorescence analysis of methanol-fixed HeLa, using DNA polymerase gamma antibody at 1: 200 dilution.

Background

Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
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    • PPig
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