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RNF5 Antibody

37869
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  Catalog No. Package Size Price
37869 100ul $279.00

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Description
  • Product Name RNF5 Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antigen affinity purification.
  • Applications WB
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous levels of total RNF5 protein.
  • Immunogen Type Peptide
  • Immunogen Description Synthetic peptide corresponding to residues near the N terminal of human ring finger protein 5, E3 ubiquitin protein ligase
  • Target Name RNF5
  • Alternative Names RMA1; RING5
  • Accession No. Swiss-Prot#: Q99942
    NCBI Gene ID: 6048
    Gene Accssion: NP_008844/Q99942
  • SDS-PAGE MW 20kd
  • Concentration 2.4mg/ml
  • Formulation Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:500-1:2000
Immunohistochemistry: 1:25-1:100

Images
  • RNF5 Antibody - SAB | Signalway Antibody

    Gel: 10%SDS-PAGE
    Lysates (from left to right): Jurkat, 231 and K562 cell
    Amount of lysate: 40ug per lane
    Primary antibody: 1/200 dilution
    Secondary antibody dilution: 1/8000
    Exposure time: 3 minutes

  • RNF5 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #37869 at dilution 1/35.

Background
The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions.
    Please let us know if you have published research using #37869 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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