• Product NameTPM2 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total TPM2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionA synthetic peptide of human TPM2.
• Target NameTPM2
• Alternative Names    DA1; DA2B; NEM4; TMSB; AMCD1;
• Accession No. Swiss-Prot#: P07951
  NCBI Gene ID: 7169
• UniprotP07951
• Gene ID7169;
• SDS-PAGE MW 33kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:1000
  IHC 1:50 - 1:100
  IF 1:50 - 1:100

This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

• Western blot analysis of extracts of various cell lines, using TPM2 at 1:400 dilution.
• Immunohistochemistry of paraffin-embedded rat heart using TPM2 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human stomach using TPM2 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human breast cancer using TPM2 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse heart using TPM2 at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting