• Product NameWNT2 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC
• Species ReactivityHuman,Rat
• SpecificityThe antibody detects endogenous level of total WNT2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human WNT2.
• Target NameWNT2
• Alternative Names    IRP; INT1L1;
• Accession No. Swiss-Prot#: P09544
  NCBI Gene ID: 7472
• UniprotP09544
• Gene ID7472;
• SDS-PAGE MW 40kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200

This gene is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Alternatively spliced transcript variants have been identified for this gene.

• Western blot analysis of extracts of SKOV3 cells, using WNT2 at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
• Immunohistochemistry of paraffin-embedded rat brain using WNT2 at dilution of 1:200 (40x lens).
• Immunohistochemistry of paraffin-embedded human liver damage using WNT2 at dilution of 1:200 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting