• Product NamePTRH2 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total PTRH2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human PTRH2.
• Target NamePTRH2
• Alternative Names    BIT1; PTH2; CFAP37; CGI-147;
• Accession No. Swiss-Prot#: Q9Y3E5
  NCBI Gene ID: 51651
• UniprotQ9Y3E5
• Gene ID51651;
• SDS-PAGE MW 19kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:10 - 1:100

The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.

• Immunohistochemistry of paraffin-embedded rat brain using PTRH2 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse spinal cord using PTRH2 at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of MCF-7 cells using PTRH2 . Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using PTRH2 at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting