• Product NameSMG8 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total SMG8 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human SMG8
• Target NameSMG8
• Alternative Names    C17orf71
• Accession No. Swiss-Prot#:Q8ND04
  NCBI Gene ID:55181
  Gene Accssion:NP_060619
• UniprotQ8ND04
• Gene ID55181;
• Calculated MW 110 kDa
• Concentration 0.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20C

• Western blotting:1:200-1000
  

Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited by release factors to stalled ribosomes together with SMG1 and SMG9 (forming the SMG1C protein kinase complex) and, in the SMG1C complex, is required to mediate the recruitment of SMG1 to the ribosome:SURF complex and to suppress SMG1 kinase activity until the ribosome:SURF complex locates the exon junction complex (EJC). Acts as a regulator of kinase activity.

• Gel: 6%SDS-PAGE
  Lysate: 40 μg, Lane: TM4 cell lysate
  Primary antibody:SMG8 Antibody at dilution 1/200
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 10 minutes

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting