• Product NameSEMA4G Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total SEMA4G protein.
• Immunogen TypeProtein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G
• Target NameSEMA4G
• Accession No. Swiss-Prot:Q9NTN9
  Gene Accssion:BC128579
• UniprotQ9NTN9
• Gene ID57715;
• SDS-PAGE MW 91KD
• Concentration 1.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:100-1:200

Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7.?

• Gel: 6%SDS-PAGE
  Lysate: 40ug Human normal colon tissuePrimary antibody: 1/600 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 5 seconds
• Immunohistochemical analysis of paraffin-embedded Human Colorectal cancer tissue using #40331 at dilution 1/100.
• Immunohistochemical analysis of paraffin-embedded HumanEsophagus cancer tissue using #40331 at dilution 1/100.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting