• Product NameCRISPR-Cas9 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationImmunogen affinity purified
• ApplicationsWB
• Immunogen Descriptionpeptide
• Alternative Names    Cas9 antibody
  CRISPR-associated endonuclease Cas9/Csn1 antibody
  CRISPR-Cas9/Csn1 antibody
  csn1 antibody
  SpyCas9 antibody
  
• Accession No. Swiss-Prot#:
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:2,000-1:5,000
  
  ICC: 1:200-1:500
  
  

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Type II system is currently the most commonly used RNA-Guided Endonuclease technology for genome engineering. There are two distinct components to this system: (1) a guide RNA and (2) an endonuclease, in this case the CRISPR associated (Cas) nuclease, Cas9. The gRNA/Cas9 complex is recruited to the target sequence by the base-pairing between the gRNA sequence and the complement to the target sequence in the genomic DNA. For successful binding of Cas9, the genomic target sequence must also contain the correct Protospacer Adjacent Motiff (PAM) sequence immediately following the target sequence.

• Western blot analysis on cas9 transfected 293 cells (+) and non transtected 293 cells (-).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting