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ME1 antibody

22785
Review: comment rank 5 pdf
  Catalog No. Package Size Price
22785 100ul $469.00

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Description
  • Product Name ME1 antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Purified by antigen-affinity chromatography.
  • Applications WB IF
  • Species Reactivity Hu
  • Immunogen Type Recombinant protein
  • Immunogen Description Recombinant protein fragment contain a sequence corresponding to a region within amino acids 161 and 489 of ME1
  • Target Name ME1
  • Accession No. Swiss-Prot:P48163
    Gene ID:4199
  • Concentration 1mg/ml
  • Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
  • Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details

Predicted MW: 64kd
Western blotting: 1:500-1:3000
Immunofluorescence: 1:100-1:200

Images
  • ME1 antibody - SAB | Signalway Antibody

    Sample(30 ug whole cell lysate)
    A: A431
    B: HeLa S3
    7.5% SDS PAGE
    Primary antibody diluted at 1: 500

  • ME1 antibody - SAB | Signalway Antibody

    Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ME1 antibody at 1: 200 dilution.

Background
This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq]
    Please let us know if you have published research using #22785 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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