• Product NameCOMMD10 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsIHC WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total COMMD10 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human COMMD10
• Target NameCOMMD10
• Alternative Names    PTD002
• Accession No. Swiss-Prot#: Q9Y6G5
  NCBI Gene ID: 51397
• UniprotQ9Y6G5
• Gene ID51397;
• Calculated MW 23kd
• Concentration 0.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  Immunohistochemistry: 1: 20-100

May modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes (PubMed:21778237). May down-regulate activation of NF-kappa-B (PubMed:15799966).

• Gel: 12%SDS-PAGE
  Lysate: 40 μg, Lane 1-3: HEPG2,293T and Hela cell lysates,
  Primary antibody:COMMD10 antibody at dilution 1/250,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 1 minute
• The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using COMMD10 Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using COMMD10 Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting