• Product NameZPBP Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsIHC WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ZPBP protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human ZPBP
• Target NameZPBP
• Alternative Names    ZPBP1
• Accession No. Swiss-Prot#: Q9BS86
  NCBI Gene ID: 11055
• UniprotQ9BS86
• Gene ID11055;
• Calculated MW 40kd
• Concentration 0.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  Immunohistochemistry: 1: 20-100

ZPBP is one of several proteins that are thought to participate in secondary binding between acrosome-reacted sperm and the egg-specific extracellular matrix, the zona pellucida (McLeskey et al., 1998 [PubMed 9378618]). May be implicated in gamete interaction during fertilization.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg, Lane: Human testis tissue lysate,
  Primary antibody:ZPBP antibody at dilution 1/200,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 30 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using ZPBP Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting