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DNA PKcs(Phospho-Thr2609) Antibody

11167
Review: comment rank 5 pdf
  Catalog No. Package Size Price
11167-1 50ul $189.00
11167-2 100ul $279.00

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Description
  • Product Name DNA PKcs(Phospho-Thr2609) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB
  • Species Reactivity Hu
  • Specificity The antibody detects endogenous level of DNA PKcs only when phosphorylated at threonine 2609.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of threonine 2609 (V-E-T(p)-Q-A) derived from Human DNA-PK.
  • Target Name DNA PKcs
  • Modification Phospho
  • Alternative Names DNPK1; PRKD; PRKDC; XRCC7; P460
  • Accession No. Swiss-Prot: P78527
    NCBI Protein: NP_001075109.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 450kd
Western blotting: 1:500~1:1000

Images
  • DNA PKcs(Phospho-Thr2609) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from K562 cells untreated(lane 1) or treated with hydroxyurea(lane 2) using DNA PKcs(Phospho-Thr2609) antibody #11167.

Background
The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties

Chan DW, et al. (2002) Genes Dev. Sep 15; 16(18): 2333-2338
Ding Q, et al. (2003) Mol Cell Biol. Aug; 23(16): 5836-5848
Douglas P, et al. (2002) Biochem J. Nov 15; 368(Pt 1): 243-251
    Please let us know if you have published research using #11167 so that we can cite your reference.
Customer Reviews and Rankings
  • Anonymous user
    05-14-2014 10:07:54

    Hi,
    Can I use it against mouse cells?

    Please, let me know ASAP.
    Thanks,
    OM

    AdministratorHi OM. Thanks for your inquiry. Sorry for late reply. The antigen is a peptide from human protein and has 77% of homology with mouse. The antibody has not been validated on mouse cells.

  • Anonymous user
    05-14-2014 10:07:42

    Hi,
    Can I use it against mouse cells?

    Please, let me know ASAP.
    Thanks,
    OM

    AdministratorHi OM. Thanks for your inquiry. Sorry for late reply. The antigen is a peptide from human protein and has 77% of homology with mouse. The antibody has not been validated on mouse cells.

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Protocol
Browsing historyclear
Note
    Application:
  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control
    Species Reactivity:
  • HuHuman
  • MsMouse 
  • RtRat 
  • Dm Drosophila melanogaster
  • C Caenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • B Bovine 
  • D Dog
  • PPig
  • HmHamster
  • ChHm Chinese Hamster 
  • ChkChicken  
  • ShpSheep  
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