• Product NamePDE6D Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human PDE6D (NP_002592.1).
• Alternative Names    PDE6D; JBTS22; PDED; phosphodiesterase 6D
• Accession No. Swiss-Prot#:O43924
  NCBI Gene ID:5147
• UniprotO43924
• Gene ID5147;
• Calculated MW 14-15kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:100
  IF 1:50 - 1:100

This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants.

• Western blot analysis of extracts of various cell lines, using PDE6D at 1:1000 dilution.
• Immunofluorescence analysis of L929 cells using PDE6D Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of U-2 OS cells using PDE6D Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting