• Product NameMAP3K1 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic synthetic peptide of human MAP3K1 (NP_005912.1).
• Alternative Names    MAP3K1; MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6; mitogen-activated protein kinase kinase kinase 1
• Accession No. Swiss-Prot#:Q13233
  NCBI Gene ID:4214
• UniprotQ13233
• Gene ID4214;
• Calculated MW 164kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:100
  IF 1:50 - 1:100

The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus.

• Western blot analysis of extracts of various cell lines, using MAP3K1 at 1:1000 dilution.
• Immunofluorescence analysis of L929 cells using MAP3K1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded rat liver using MAP3K1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human liver using MAP3K1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse kidney using MAP3K1 at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting