• Product NameSMPD1 / ASM Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant protein of human SMPD1 / ASM
• Alternative Names    SMPD1; ASM; ASMASE; NPD; sphingomyelin phosphodiesterase
• Accession No. Swiss-Prot#:P17405
  NCBI Gene ID:6609
• UniprotP17405
• Gene ID6609;
• Calculated MW 60kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:50 - 1:100

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified.

• Immunofluorescence analysis of NIH-3T3 cells using SMPD1 / ASM Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of THP-1 cells, using SMPD1 / ASM at 1:1000 dilution. THP-1 cells were treated by PMA (80 nM) for overnight.
• Western blot analysis of extracts of various cell lines, using SMPD1 / ASM at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting