• Product NameHeparanase 1 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant protein of human Heparanase 1
• Alternative Names    HPSE; HPA; HPA1; HPR1; HPSE1; HSE1; heparanase
• Accession No. Swiss-Prot#:Q9Y251
  NCBI Gene ID:10855
• UniprotQ9Y251
• Gene ID10855;
• Calculated MW 50kDa/65kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.

• Western blot analysis of extracts of various cells, using Heparanase 1 at 1:1000 dilution.
• Immunofluorescence analysis of HeLa cells using Heparanase 1 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of NIH/3T3 cells using Heparanase 1 at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting