• Product NameBeclin 1 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human Beclin 1 (NP_003757.1).
• Alternative Names    BECN1; ATG6; VPS30; beclin1; beclin-1
• Accession No. Swiss-Prot#:Q14457
  NCBI Gene ID:8678
• UniprotQ14457
• Gene ID8678;
• Calculated MW 60kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200
  IP 1:50 - 1:100

This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.

• Immunofluorescence analysis of HeLa cells using Beclin 1 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using Beclin 1 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded human stomach using Beclin 1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse kidney using Beclin 1 at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting