• Product NameSMAD2/SMAD3 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human SMAD3 (NP_005893.1).
• Alternative Names    HSPC193;HsT17436;JV15-2;LDS1C;LDS3;MADH3
• Accession No. Swiss-Prot#:P84022/Q15796
  NCBI Gene ID:4087/4088
• UniprotP84022
• Gene ID4088;
• Calculated MW 56-60kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

• Immunofluorescence analysis of C6 cells using SMAD2 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of NIH-3T3 cells using SMAD2 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of NIH-3T3 cells using SMAD2 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of U-2 OS cells using SMAD2 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of C6 cells using SMAD2 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of NIH-3T3 cells using SMAD2 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of U-2 OS cells using SMAD2 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of U-2 OS cells using SMAD2 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of C6 cells using SMAD2 at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting