• Product NameIRAK-M Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms Rt
• SpecificityIRAK-M antibody has no cross response to IRAK or IRAK2.
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a peptide corresponding to amino acids from the carboxy terminus of human IRAK-M.
• Target NameIRAK-M
• Accession No. Swiss-Prot:Q9Y616
  Gene ID:11213
• UniprotQ9Y616
• Gene ID11213;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Predicted MW: 68 kd
  

Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor-associated kinase (IRAK and IRAK2) mediates the activation of NF-betaB by IL-1/Toll receptors, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-M. IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and the dominant negative mutants of IRAKs inhibit IL-1 or LPS induced NF-betaB activation. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses to cytokine IL-1 and LPS.

• Western blot analysis of IRAK-M in mouse spleen (M) and rat liver (R) tissue lysates with IRAK-M antibody at 1 ug/mL.
• Immunohistochemical staining of rat liver cells using IRAK-M antibody at 2 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting