• Product NameRhoA Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human RhoA (NP_001655.1).
• Alternative Names    ARH12;ARHA;RHO12;RHOH12;Rho;RHOA;RhoA
• Accession No. Swiss Prot:P61586
  GeneID:387
• UniprotP61586
• Calculated MW 21kDa
• SDS-PAGE MW 21kDa
• Formulation Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified.

• Immunohistochemistry of paraffin-embedded rat brain using RhoA at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human gastric cancer using RhoA at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse brain using RhoA at dilution of 1:100 (40x lens).
• Western blot analysis of extracts of various cell lines, using RhoA at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting