• Product NameTRIM28 Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human TRIM28 (NP_005753.1).
• Alternative Names    KAP1;PPP1R157;RNF96;TF1B;TIF1B;TRIM28
• Accession No. Swiss Prot:Q13263
  GeneID:10155
• UniprotQ13263
• Calculated MW 79kDa/88kDa
• SDS-PAGE MW 120kDa
• Formulation Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:1000 - 1:3000
  IHC 1:100 - 1:200
  IF 1:50 - 1:200

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

• Immunofluorescence analysis of C6 cells using TRIM28 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of L929 cells using TRIM28 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of U-2 OS cells using TRIM28 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using TRIM28 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded rat brain using TRIM28 at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting