• Product NameGOT2 Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human GOT2 (NP_002071.2).
• Alternative Names    GOT2;KAT4;KATIV;KYAT4;mitAAT
• Accession No. Swiss Prot:P00505
  GeneID:2806
• UniprotP00505
• Calculated MW 43kDa/47kDa
• SDS-PAGE MW 48kDa
• Formulation Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene.

• Immunohistochemistry of paraffin-embedded rat kidney using GOT2 at dilution of 1:100 (40x lens).
• Western blot analysis of extracts of various cell lines, using GOT2 at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting