• Product NameZBED3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB ICC
• Species ReactivityHu
• SpecificityThis antibody is predicted to have no cross-reactivity to ZBED1 or ZBED2.
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a 15 amino acid peptide from near the carboxy terminus of human ZBED3.
• Target NameZBED3
• Alternative Names    Zinc finger BED-type domain containing 3
• Accession No. Swiss-Prot:Q96IU2
  Gene ID:84327
• UniprotQ96IU2
• Gene ID84327;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Axin is a key modulator of the Wnt/beta-catenin pathway and acts as a scaffold protein in phosphorylating and degrading cytoplasmic beta-catenin. ZBED3, a member of the Zinc finger BED-type domain containing superfamily, has been recently identified as an Axin-interacting protein and has been suggested to modulate the Wnt/beta-catenin signaling pathway. RNA interference (RNAi) experiments targeting ZBED3 RNA attenuated Wnt-induced beta-catenin accumulation, lymphoid enhancer binding factor-1-dependent luciferase reporter activity, and Wnt gene expression.

• Western blot analysis of ZBED3 in human lung tissue lysate with ZBED3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
• Immunocytochemistry of ZBED3 in A549 cells with ZBED3 antibody at 20 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting