• Product NameCDK6 Rabbit mAb
• Clone No.S03-2G5
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB;IHC;ICC/IF;FC
• Species ReactivityHuman
• Immunogen DescriptionA synthesized peptide derived from human CDK6
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    MCPH12; PLSTIRE
• Accession No. Swiss-Prot:Q00534
  GeneID:1021
• UniprotQ00534
• Gene ID1021
• Calculated MW Calculated MW: 37 kDa; Observed MW: 37 kDa
• Concentration 0.3 mg/ml
• Formulation Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

• WB 1:1000-1:5000;
  IHC 1:100-1:200;
  ICC/IF 1:50-1:200;
  FC 1:20-1:100

Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle.

• All lanes use the Antibody at 1:5000 dilution for 1 hour at room temperature.
• Immunohistochemical analysis of paraffin-embedded human lung cancer, using CDK6 Antibody.
• Immunofluorescent analysis of K562 cells, using CDK6 Antibody.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
• Immunofluorescent analysis using the Antibody at 1:50 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting