• Product NameInsulin Receptor Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB ICC/IF
• Species ReactivityHuman Rat
• SpecificityInsulin Receptor Antibody detects endogenous levels of Insulin Receptor
• Immunogen DescriptionA synthesized peptide derived from human Insulin Receptor
• Alternative Names    CD220; HHF5; human insulin receptor; Insr; Insulin receptor subunit beta; IR 1; IR;
• Accession No. Uniprot:P06213
• UniprotP06213
• Calculated MW 95kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:2000 ICC/IF 1:50~1:200

• Insulin Receptor is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues; Insulin binding to IR induces phosphorylation of intracellular tyrosine kinase domains and recruitment of multiple SH2 and SH3 domain-containing intracellular proteins that serve as signaling intermediates for pleiotropic effects of insulin.

Insulin Receptor is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues; Insulin binding to IR induces phosphorylation of intracellular tyrosine kinase domains and recruitment of multiple SH2 and SH3 domain-containing intracellular proteins that serve as signaling intermediates for pleiotropic effects of insulin.

• Western blot analysis of Insulin Receptor expression in HeLa cell lysate.
• Immunofluorescent analysis of BxPC-3 cells, using Insulin Receptor Antibody.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting