• Product NameRBM35A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms Rt
• SpecificityRBM35A antibody is predicted to not cross-react with other RBM35/ESRP family members. At least five isoforms of RBM35A are known to exist; this antibody will detect all five.
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a 18 amino acid peptide near the amino terminus of human RBM35A.
• Target NameRBM35A
• Alternative Names    RNA-binding motif protein 35A, RMB35A, Epithelial splicing regulatory protein 1, ESRP1
• Accession No. Swiss-Prot:Q6NXG1
  Gene ID:54845
• UniprotQ6NXG1
• Gene ID54845;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

RBM35A, also known as ESRP1, is a mRNA splicing factor that with its related protein RBM35B (ESRP2) are coordinators of an epithelial cell-type-specific splicing program. RBM35A contains three putative RNA recognition motifs and acts by directly binding specific sequences in mRNAs. RBM35A is involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs. Other recent studies have shown that RMB35A may also act as a novel tumor suppressor.

• Western blot analysis of RBM35A in rat colon tissue lysate with RBM35A antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.
• Immunohistochemistry of RBM35A in human colon tissue with RBM35A antibody at 2.5 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting