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CYP1A1 Antibody

31065
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  Catalog No. Package Size Price
31065-1 50ul $189.00
31065-2 100ul $279.00

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Description
  • Product Name CYP1A1 Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Applications ELISA WB IHC
  • Species Reactivity Hu Ms
  • Specificity The antibody detects endogenous level of total CYP1A1 protein.
  • Immunogen Type Recombinant Protein
  • Immunogen Description Fusion protein corresponding to C terminal 300 amino acids of human cytochrome P450, family 1, subfamily A, polypeptide 1
  • Target Name CYP1A1
  • Alternative Names cytochrome P450, family 1, subfamily A, polypeptide 1, AHH, AHRR, CP11, CYP1, P1-450, P450-C, P450DX
  • Accession No. Genbank No.: BC023019
  • Formulation Supplied at 0.8mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
  • Storage Store at -20˚C/1 year
Application Details

Predicted MW: 58kd
ELISA: 1:1000-1:5000
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:25-1:100

Images
  • CYP1A1 Antibody - SAB | Signalway Antibody

    Gel: 10%SDS-PAGE
    Lysate: 30 µg Mouse liver tissue lysate
    Primary antibody: 1/400 dilution
    Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
    Exposure time: 5 seconds

  • CYP1A1 Antibody - SAB | Signalway Antibody

    The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 31065(CYP1A1 Antibody) at dilution 1/25, on the right is treated with the fusion protein.

Background

This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15.

    Please let us know if you have published research using #31065 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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