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BRAF Antibody

31224
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  Catalog No. Package Size Price
31224-1 50ul $189.00
31224-2 100ul $279.00

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Description
  • Product Name BRAF Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Applications ELISA WB IHC
  • Species Reactivity Hu Ms
  • Specificity The antibody detects endogenous level of total BRAF protein.
  • Immunogen Type Peptide
  • Immunogen Description Synthetic peptide corresponding to a region derived from 591-607 amino acids of Human v-raf murine sarcoma viral oncogene homolog B1
  • Target Name BRAF
  • Alternative Names V-raf murine sarcoma viral oncogene homolog B1, NS7, BRAF1, RAFB1, B-RAF1
  • Accession No. Genbank No.: NP_004324
  • Formulation Supplied at 2.1mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
  • Storage Store at -20˚C/1 year
Application Details

Predicted MW: 84kd
ELISA: 1:1000-1:5000
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:50-1:200

Images
  • BRAF Antibody - SAB | Signalway Antibody

    Gel: 8%SDS-PAGE
    Lane1: Hela cell lysate
    Lane2: HT-29 cell lysate
    Lysates: 50 ug per lane
    Primary antibody: 1/1400 dilution
    Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
    Exposure time: 5 minutes

  • BRAF Antibody - SAB | Signalway Antibody

    The image on the left is immunohistochemistry of paraffin-embedded human tonsil tissue using 31224 (BRAF Antibody) at dilution 1/60, on the right is treated with the synthetic peptide.

Background
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
    Please let us know if you have published research using #31224 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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