• Product NameSMARCB1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total SMARCB1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human SMARCB1.
• Target NameSMARCB1
• Alternative Names    RDT; INI1; SNF5; Snr1; BAF47
• Accession No. Swiss-Prot:Q12824
  NCBI Gene ID:6598
• UniprotQ12824
• Gene ID6598;
• SDS-PAGE MW 44KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene.

• Western blot analysis of extracts of various cell lines, using SMARCB1 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded mouse osteosarcoma using SMARCB1 at dilution of 1:200 (40x lens).
• Immunohistochemistry of paraffin-embedded rat Intestine using SMARCB1 at dilution of 1:200 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting