• Product NamePKA-R2β (Ab-113) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total PKA-R2β protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized non-phosphopeptide derived from human PKA-R2β around the phosphorylation site of serine 113 (R-A-S(p)-V-C).
• Target NamePKA-R2β
• Alternative Names    KAP3; PKA R2-beta; PRKAR2B; cAMP-dependent protein kinase type II-beta regulatory chain;
• Accession No. Swiss-Prot: P31323
  NCBI Gene ID: 5577
• UniprotP31323
• Gene ID5577;
• SDS-PAGE MW 46kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

Levy F.O., Mol. Endocrinol. 2:1364-1373(1988).
Ota T., Nat. Genet. 36:40-45(2004).
Scherer S.W., Science 300:767-772(2003)

• Western blot analysis of extracts from HepG2 cells and K562 cells, using PKA-R2β (Ab-113) antibody #33161.
• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PKA-R2β (Ab-113) antibody #33161.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting