• Product NameWNT1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total WNT1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human WNT1.
• Target NameWNT1
• Alternative Names    ONCOGENE INT1; wingless-type MMTV integration site family; member 1; INT1; Proto-oncogene protein Wnt-1 [Precursor]
• Accession No. Swiss-Prot: P04628
  NCBI Gene ID: 7471
• UniprotP04628
• Gene ID7471;
• SDS-PAGE MW 45kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Has a role in osteoblast function and bone development.

van Ooyen A., EMBO J. 4:2905-2909(1985).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

• Western blot analysis of extracts from HT-29 cells, using WNT1 antibody #33543.
• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using WNT1 antibody #33543.
• Immunofluorescence analysis of COS-7 cells, using WNT1 antibody #33543.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting