• Product NameCDC7 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CDC7 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human CDC7.
• Target NameCDC7
• Alternative Names    Cell division cycle 7-related protein kinase; EC 2.7.11.1; CDC7-related kinase; HsCdc7; huCdc7
• Accession No. Swiss-Prot: O00311
  NCBI Gene ID: 8317
• UniprotO00311
• Gene ID8317;
• SDS-PAGE MW 70kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.

Sato N., EMBO J. 16:4340-4351(1997).
Hess G.F., Gene 211:133-140(1998).
Jiang W., Proc. Natl. Acad. Sci. U.S.A. 94:14320-14325(1997).

• Western blot analysis of extracts from Jurkat cells, using CDC7 antibody #33558.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting