• Product NameHistone 1F0 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Histone 1F0 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human Histone 1F0.
• Target NameHistone 1F0
• Alternative Names    H1; H1 histone family; member 0; H10; H1FV
• Accession No. Swiss-Prot: P07305
  NCBI Gene ID: 3005
• UniprotP07305
• Gene ID3005;
• SDS-PAGE MW 20kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.

Doenecke D., J. Mol. Biol. 187:461-464(1986).
Collins J.E., Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004).
Dunham I., Nature 402:489-495(1999).

• Western blot analysis of extracts from A549 cells, using Histone 1F0 antibody #33655.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting