• Product NameMARK3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total MARK3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from N-terminal of human MARK3.
• Target NameMARK3
• Alternative Names    C-TAK1; Cdc25C- associated protein kinase 1; EC 2.7.11.1; MAP/microtubule affinity-regulating kinase 3; Protein kinase STK10
• Accession No. Swiss-Prot: P27448
  NCBI Gene ID: 4140
• UniprotP27448
• Gene ID4140;
• SDS-PAGE MW 85kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Involved in the specific phosphorylation of microtubule-associated proteins for tau, MAP2 and MAP4. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus.

Peng C.Y., Cell Growth Differ. 9:197-208(1998).
Sun T.-Q., Nat. Cell Biol. 3:628-636(2001).
Wissing J., Mol. Cell. Proteomics 6:537-547(2007)

• Western blot analysis of extracts from HeLa cells and 293 cells, using MARK3 antibody #33730.
• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using MARK3 antibody #33730.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting