• Product NameGRK6 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total GRK6 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from N-terminal of human GRK6.
• Target NameGRK6
• Alternative Names    EC 2.7.11.16; G-protein-coupled receptor kinase 6; GPRK6; kinase GRK6;
• Accession No. Swiss-Prot: P43250
  NCBI Gene ID: 2870
• UniprotP43250
• Gene ID2870;
• SDS-PAGE MW 65kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis By similarity. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro).

Benovic J.L., J. Biol. Chem. 268:19521-19527(1993).
Haribabu B., Proc. Natl. Acad. Sci. U.S.A. 90:9398-9402(1993).
Stoffel R.H., J. Biol. Chem. 269:27791-27794(1994).

• Western blot analysis of extracts from Jurkat cells, COLO205 cells and HUVEC cells, using GRK6 antibody #33771.
• Immunofluorescence analysis of HeLa cells, using GRK6 antibody #33771.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting