• Product NameSMG7 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total SMG7 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human SMG7.
• Target NameSMG7
• Alternative Names    C1orf16; EST1C; KIAA0250;
• Accession No. Swiss-Prot: Q92540
  NCBI Gene ID: 9887
• UniprotQ92540
• Gene ID9887;
• SDS-PAGE MW 127kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation.

Ohnishi T., Mol. Cell 12:1187-1200(2003).
Nagase T., DNA Res. 3:321-329(1996).
Nakajima D., DNA Res. 9:99-106(2002).

• Western blot analysis of extracts from HeLa cells, Jurkat cells, 293 cells and HUVEC cells, using SMG7 antibody #34002.
• Immunofluorescence analysis of HepG2 cells, using SMG7 antibody #34002.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting