• Product NameDUSP16 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total DUSP16 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human DUSP16.
• Target NameDUSP16
• Alternative Names    Dual specificity protein phosphatase 16; EC 3.1.3.48; EC 3.1.3.16; Mitogen-activated protein kinase phosphatase 7; MAP kinase phosphatase 7
• Accession No. Swiss-Prot: Q9BYG9
  NCBI Gene ID: 80824
• UniprotQ9BY84
• Gene ID80824;
• SDS-PAGE MW 73kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

This gene encodes a mitogen-activated protein kinase phosphatase that is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. The encoded protein specifically regulates the c-Jun amino-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) pathways.

Masuda K., J. Biol. Chem. 276:39002-39011(2001).
Hoornaert I., Oncogene 22:7728-7736(2003).
Nagase T., DNA Res. 7:347-355(2000).

• Western blot analysis of extracts from Jurkat cells and COLO205 cells, using DUSP16 antibody #34049.
• Immunofluorescence analysis of HepG2 cells, using DUSP16 antibody #34049.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting