• Product NameIRX2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total IRX2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human IRX2.
• Target NameIRX2
• Alternative Names    Iroquois-class homeodomain protein IRX-2; Iroquois homeobox protein 2; Homeodomain protein IRXA2; IRX2; IRXA2
• Accession No. Swiss-Prot: Q9BZI1
  NCBI Gene ID: 153572
• UniprotQ9BZI1
• Gene ID153572;
• SDS-PAGE MW 50kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

IRX2 is a member of the Iroquois homeobox gene family. Members of this family appear to play multiple roles during pattern formation of vertebrate embryos.[supplied by OMIM, Apr 2004]

Hansen L., Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Ogura K., Cytogenet. Cell Genet. 92:320-325(2001).

• Western blot analysis of extracts from 293 cells and HepG2 cells, using IRX2 antibody #34053.
• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using IRX2 antibody #34053.
• Immunofluorescence analysis of HepG2 cells, using IRX2 antibody #34053.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting