• Product NameDOK5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total DOK5 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human DOK5.
• Target NameDOK5
• Alternative Names    Docking protein 5; Downstream of tyrosine kinase 5; Insulin receptor substrate 6; IRS-6; IRS6
• Accession No. Swiss-Prot: Q9P104
  NCBI Gene ID: 55816
• UniprotQ9P104
• Gene ID55816;
• SDS-PAGE MW 36kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK5 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of RET in neuronal differentiation.

Luo W.Q., Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
Favre C., Genes Immun. 4:40-45(2003).
Cai D., J. Biol. Chem. 278:25323-25330(2003).

• Western blot analysis of extracts from K562 cells, using DOK5 antibody #34089.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting