• Product NameDBF4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total DBF4 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from N-terminal of human DBF4.
• Target NameDBF4
• Alternative Names    Protein DBF4 homolog A; Activator of S phase kinase; DBF4-type zinc finger-containing protein 1; Chiffon homolog A; DBF4
• Accession No. Swiss-Prot: Q9UBU7
  NCBI Gene ID: 10926
• UniprotQ9UBU7
• Gene ID10926;
• SDS-PAGE MW 77kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.

Kumagai H., Mol. Cell. Biol. 19:5083-5095(1999).
Jiang W., EMBO J. 18:5703-5713(1999).
Ota T., Nat. Genet. 36:40-45(2004).

• Western blot analysis of extracts from NIH-3T3 cells treated with H2O2 (100uM, 30mins) and COS-7 cells treated with PMA (125mg/ml, 30mins), using DBF4 antibody #34095.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting