• Product NameCDC20 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total CDC20 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human CDC20.
• Target NameCDC20
• Alternative Names    CC20; Cell division cycle protein 20 homolog; p55CDC;
• Accession No. Swiss-Prot: Q12834
  NCBI Gene ID: 991
• UniprotQ12834
• Gene ID991;
• SDS-PAGE MW 50kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.

Weinstein J., Mol. Cell. Biol. 14:3350-3363(1994).
Kramer E.R., Curr. Biol. 8:1207-1210(1998).
Gregory S.G., Nature 441:315-321(2006).

• Western blot analysis of extracts from HeLa cells and Jurkat cells, using CDC20 antibody #34189.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting