• Product NameCollagen I α2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Collagen I α2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human Collagen I α2.
• Target NameCollagen I α2
• Alternative Names    Collagen alpha-2(I) chain [Precursor]; Alpha-2 type I collagen; CO1A2;
• Accession No. Swiss-Prot: P08123
  NCBI Gene ID: 1278
• UniprotP08123
• Gene ID1278;
• SDS-PAGE MW 125kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Type I collagen is a member of group I collagen (fibrillar forming collagen).

Dalgleish R., Nucleic Acids Res. 25:181-187(1997).
Korkko J.M., Am. J. Hum. Genet. 62:98-110(1998).
Akai J., Connect. Tissue Res. 30:1-6(1998).

• Western blot analysis of extracts from 293 cells and HeLa cells, using Collagen I α2 antibody #34203.
• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Collagen I α2 antibody #34203.
• Immunofluorescence analysis of HeLa cells, using Collagen I α2 antibody #34203.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting