• Product NameCyclosome 1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total Cyclosome 1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human Cyclosome 1.
• Target NameCyclosome 1
• Alternative Names    ANAPC1; ANC1; Anaphase promoting complex subunit 1; Cyclosome subunit 1; Mitotic checkpoint regulator
• Accession No. Swiss-Prot: Q9H1A4
  NCBI Gene ID: 64682
• UniprotQ9H1A4
• Gene ID64682;
• SDS-PAGE MW 22kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• 
  
  Immunofluorescence: 1:100~1:500

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Joergensen P.M., Gene 262:51-59(2001).
Ota T., Nat. Genet. 36:40-45(2004).
Kraft C., EMBO J. 22:6598-6609(2003).

• Immunofluorescence analysis of HepG2 cells, using Cyclosome 1 antibody #34257.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting